Friday, January 24, 2020

Marketing changes :: essays research papers

There have been many significant changes within our society. One of this is the changing gender roles within our society and another is the changing attitudes toward health & fitness. Both of these have impacted on consumer preferences or buying habits. Gender roles are no longer as accurate as it once was. There is now a trend of reserved roles. This is due to the continued growth in a number of dual income households. There has been an increase of women in the workforce in the fast few years. Women in the paid workforce spend less time shopping than the stay at home women. They tend to shop in the evenings and on weekends due to their busy schedules. They tend to swap stores which they shop in due to convenience. This happens due to the time constraints but still earning an income they are less likely to be price conscious. The working women are more likely to purchase convenience foods and products and they tend to be brand and store loyal. In this current society women have different material (clothing) needs to the stay at home women versus the working women. Males are now also more involved in purchasing household products. Some men now do their shopping via the internet rather than physically enter the stores. Because they use this type of method it is harder to record their purchasing habits. They have moved away from traditional roles. Women are now taking a greater role in deciding which products to buy. Buying decisions have moved away from being male/husband denominated to become automatic. i.e. many families now have two cars and they tend to make their purchase decision separate. Direct marketers are now targeting time pressured women who use catalogues, telesales and internet sites as ways of shopping may it be for clothing as well as household and family needs. They have also used this method to target men who now also do the shopping. Marketers who are aware of this change have developed changes to their product design and advertising appeal/styles. Some advertisements have been positive and some offending. I.e. Detergent Ads. Marketers are increasingly attempting to cash in on the belief that men are getting in touch with their feminine side. Many businesses have now marketed their services/ products to suit these changing gender roles. One of this is restaurants. Plenty of them now offer home delivery service. There is also an increase in takeaway stores catering for these busy men and women.

Wednesday, January 15, 2020

Blue White Selection

Blue white selection  is a widely used method in screening recombinants in cloning. This is based on the gene product of lac z gene. The plasmid vectors contain this gene which produces  ? galactosidase  enzyme. When a gene is inserted close to  lac z gene, the reading frame will be distorted and the gene is inactivated. So the transformed cells will not produce this enzyme and are called competent cells. After the recombination, the bacterial cells are grown in a medium containing X gal (5-bromo-4-chloro-indolyl-? D-galactopyranoside) and  IPTG (Isopropyl ? -D-1-thiogalactopyranoside). IPTG acts as the inducer for lac z gene and enhance the production of ? galactosidase. When it is produced, combines with X gal to form a blue colour complex called  5,5†²-dibromo-4,4†²-dichloro-indigo  which is insoluble. The transformed colonies will appear white in colour and non- transformed cells will appear blue in colour. This method is also called as insertional inactiv ation of lac z gene. Hybridization  techniques are widely used to identify recombinants.This is based on the ability of nucleic acids hybridize with complementary DNA. The transformed cells are transferred on to a  nitrocellulose membrane  which is subjected to cell lysis. The double stranded DNA is converted to single stranded DNA and immobilized on the membrane. Then it is treated with radiolabelled  probes  complementary to target DNA. If the desired DNA is present, the probes will be hybridized which can be detected by autoradiography. Apart from these methods,  immunochemical methods  are used to detect protein products to screen recombinants.

Tuesday, January 7, 2020

America Needs More Government Programs to Pull Children...

The face of poverty is changing in the United States. When someone mentions that a person is living in poverty, we are inclined to think of a bum living on the street, eating at shelters, and using whatever money they have for alcohol, drugs and tobacco. Usually we visualize this person as being a middle-aged male with drab clothing and a long beard. This description may have fit the average person living in poverty or on the street a few years ago, but it is no longer a correct generalization. Children are the face of poverty in 2015. These children have little chance to rise up out of the lifestyle they are born into. Many of these children are born to teenage girls. The economic states of these mothers and their lifestyles many times†¦show more content†¦Some people are born into stable families, so they have m ore opportunities than those that are born into poverty. No matter how people feel toward the choices that the parents made, we cant hold this against the child. One other thing society canà ­t do is simply hand out money or food stamps to the families of these children. Who knows where this money is going or what it is being used for? The food stamps could be intended to feed a baby, but instead they could be used to buy food or items that a child will never see. It is also all to common for parents living in poverty or on the streets to use what money they get for alcohol or cigarettes. Instead of handing out money to these people, they should be put i n treatment programs that could help them fight their addictions. This would be of great benefit to their children now and in the future. Not all mothers and fathers in need of assistance are going to be irresponsible with what they are given, but we ca n not take the chance of having the money wasted and the children neglected. Who wants the money that they worked for going to someone else, so that person does not have to work, yet still gets paid regularly just because they had a baby when they were n ot ready? As I stated before, not all parents living in poverty are irresponsible and many have legitimate reasons for being where they are. But because there are people who will take advantage of the system, we need to watch out for the children of tho seShow MoreRelatedAmerica Needs Better Funding For The Safety Net Programs1644 Words   |  7 PagesAmerica, where the famous so-called â€Å"American Dream† was born, used to be the most influential nation in the world economy. However, since the Second World War, America’s economy has been in a free-fall. The â€Å"American Dream† is now only a remnant of long lost heydays. The reason why America has gotten to this dreadful situation may lie in the path that its government chose to develop. 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